If you’re managing a food forest or organic edible garden in Florida (and beyond), you’ve probably dealt with seemingly endless soil-based challenges. One of the sneakier enemies is the root-knot nematode. These microscopic roundworms live in the soil, infect plant roots, then create bumpy knots or “galls” that weaken plants, and lower their productivity.
Meloidogyne incognita are sedentary endoparasites. Once they invade roots, they prevent them from properly absorbing water and nutrients, leading to above-ground symptoms like stunted growth, yellowing, and wilting — and then reproduce inside the plant and in the soil. It’s as nasty as it sounds, as shown in the photo below:

Why this matters for Florida food forestry / edible gardening:
- The warm, sandy soils here in North Central Florida often harbor root-knot nematodes.
- Many of our edibles and food forest plants are susceptible hosts.
- When roots are damaged, the plant’s ability to absorb nutrients and water is compromised. This is especially problematic during our summer heat and dry spells. (Plants may wilt even when soil moisture is adequate.)
- Below the soil, nematode damage can invite secondary fungal or bacterial root rots and disease, further weakening plants.
- Because they’re hidden underground, nematodes often go unnoticed until symptoms are advanced and the yield is already impacted.

Symptoms to watch for:
- Above ground: stunted growth, yellowing of leaves, reduced vigor, lower yields.
- Below ground: swollen or knotted roots (galls) when you gently investigate, and inability of roots to spread properly.
- In heavy infestations, wilting may occur during hot or dry spells despite adequate watering.
Why conventional chemical options should be avoided:
Traditional nematicides are often chemical-heavy, carry environmental risks, may harm beneficial soil biota, and don’t align with regenerative growing. Also, nematode control in soils can be tricky due to their hidden nature and reproduction cycles. The good news is that there’s a biological control option that fits much better with a permaculture mindset.

Trichoderma: A Soil-Friendly Ally
Trichoderma harzianum (and its “cousins” in the genus Trichoderma) are fungi that live in soil and roots, often as beneficial symbionts or antagonists. They are increasingly used as biocontrol agents for fungi, root pathogens — and in this case, root-knot nematodes.
What Trichoderma does:
- It colonizes the root zone and can form relationships with plant roots, increasing nutrient uptake, promoting root growth, and improving plant vigor.
- It also has antagonistic effects: competition for space and resources, production of enzymes (like chitinases, proteases, glucanases) which degrade nematode eggs/juveniles or fungal pathogens.
- It can trigger or induce plant systemic resistance. The plant’s own defenses against root pathogens or nematodes are enhanced.
- It can directly reduce nematode populations (eggs and juveniles) in the soil and reduce root galling/infection. For example, one research showed T. harzianum induced ~61.9% reduction of RKN infestation in tomato. ScienceDirect
Why this fits permaculture & regenerative design:
- Low-toxicity, soil-biota friendly.
- Supports soil health and plant vigor rather than simply suppressing the problem.
- Compatible with other biological practices (companion planting, organic composts, minimal disturbance).
- Helps build resilience rather than relying on a chemical “fix”.

How to Use Trichoderma to Combat Root-Knot Nematodes in Your Garden or Food-Forest
1. Set up the soil environment
- Ensure good drainage and avoid compacted zones — nematodes love hot, stressed roots; healthy roots resist them better.
- Incorporate organic matter: compost, aged mulch, cover-crop residues — this supports beneficial microbes, including Trichoderma.
- Consider rotating beds or intercropping with nematode-suppressive plants (French marigolds, castor beans, sorghum-sudangrass, and sunn hemp release compounds that are toxic or suppressive to nematodes, acting as “trap crops”).
2. Choose a Trichoderma product or inoculum
- Source a reputable commercial product or local inoculum containing Trichoderma spp. (for example, T. harzianum, T. viride, T. asperellum).
- Check the label for nematode control efficacy. Research has shown strains of T. viride, T. harzianum among the more effective.
- Consider “pre-planting” application: many studies show greater success when applied before heavy nematode pressure or at the start of the cropping cycle.
- See the next section for how to make Trichoderma at home.
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3. Application timing & method
- Pre-planting: Amend your planting bed or raised bed soil with Trichoderma inoculum (granular or slurry) before seedlings or transplants go in.
- Root-dipping/seedling treatment: If you’re transplanting young edibles or trees, you can dip roots or apply a slurry of Trichoderma around the root zone.
- In-season reinforcement: After planting, you can apply Trichoderma around the root zone or as a soil drench during irrigation (especially in summer).
- Combine with organic amendments: There may be improved results when Trichoderma is combined with organic materials such as compost. Using Trichoderma with chop and drop is a synergistic approach where you use the beneficial fungus to inoculate the soil before chopping and dropping plant matter, creating a healthier soil environment to support the decomposition process.
4. Monitor and integrate with other soil-health tactics
- After 4-8 weeks, check root systems for early galling signs or root vigor.
- Maintain mulch, compost top-dressing, and avoid soil disturbance to preserve beneficial fungal networks.
- Consider rotating in nematode-resistant cultivars of edibles or tropical trees.
- Maintain proper watering.
5. Expect realistic results and remain patient
- Biocontrol is not an instant “zap” like synthetic nematicides; it works by shifting soil biology and building resilience.
- In greenhouse/field experiments, reductions in galling and nematode numbers ranged from ~30% up to ~60% or more, depending on strain, treatment method, and soil conditions.
- In a food forest, view this as part of the “system” rather than a standalone fix: soil biology + plant health + diversity + beneficial fungi = stronger ecosystem.

How to Make Trichoderma at Home
1. Get a Starter Source
You need an initial colony of Trichoderma to expand. Options:
- Buy a commercial inoculant (powder or liquid) online or at a garden store.
- Collect naturally: Trichoderma thrives on decaying wood, leaf litter, and forest soil in warm, humid places (perfect in Florida). Look for white/green fuzz on decomposing logs — that’s often Trichoderma.
2. Prepare a Growth Medium (Food for the Fungus)
Trichoderma multiplies best on carbon-rich, low-cost substrates. You can use:
- Cooked rice (classic method)
- Sterilized sorghum or millet
- Moistened coconut coir + a little molasses
Key: Substrate should be slightly moist, not soggy, and free of contaminants (boil/steam for 20–30 min if possible).
3. Inoculate
- Place your starter Trichoderma (a pinch of powder, or a small piece of colonized material) into the cooled substrate.
- Mix gently with clean hands or utensils.
- Store in a breathable container (paper bag, cardboard box, or jar covered in cloth).
4. Incubate
- Keep in a warm, shaded spot (75–85°F is perfect for Florida).
- Within 3–7 days, you should see greenish fuzz spreading through the substrate — that’s Trichoderma sporulating.
- By 10–14 days, the whole substrate should be colonized.
5. Multiply Into Liquid Form
Once you have solid inoculum, you can brew a liquid suspension for soil drenches:
- Blend a handful of colonized rice (or coir) in a 5-gallon bucket of non-chlorinated water.
- Add a spoonful of molasses or fish hydrolysate (optional food source).
- Stir daily for 3–4 days — don’t let it go anaerobic (bubblers/air stones help but aren’t required).
- Strain and apply as a soil drench or root dip.
6. Apply in Your Garden
- Soil Drench: Pour around roots of perennials, vegetables, and fruit trees.
- Seedling Dip: Coat roots in liquid inoculant before transplanting.
- Compost Booster: Add to compost piles to speed decomposition and outcompete pathogens.
Tips & Cautions
- Avoid direct sun & heat when applying — fungi prefer moist, shaded soil.
- Don’t mix with chemical fungicides — they’ll kill your Trichoderma too.
- Refresh cultures every few weeks. Homemade inoculants lose strength over time.
- Trichoderma needs organic matter in the soil to persist — keep mulching and composting.
Done right, this DIY method gives you an ongoing supply of Trichoderma for pennies, and it integrates beautifully with Florida food forest systems.

Sample Food Forest Workflow
Here’s how you might integrate Trichoderma into a bed or zone in your North Central Florida food forest:
- Bed Prep (late winter/early spring): Clear the bed, rake/light till to loosen the soil, incorporate 2–3 inches of well-aged compost, and let it settle.
- Inoculate: Apply Trichoderma inoculum across the bed. Mix it with topsoil, water it in, and lightly mulch.
- Plant: When conditions are right (seedlings or transplants), plant your edibles/perennials. Optionally dip roots or treat the root zone with a Trichoderma slurry.
- Maintain: Mulch around plants, top-dress with compost every couple of months, maintain proper irrigation, and avoid root stress.
- Monitor: After 6-8 weeks, inspect roots of a few plants (especially if symptoms appear) for galling. Note plant vigor, yield, etc.
- Supplement action: If you find moderate galling, next cycle consider adding a host-break crop or nematode-resistant cultivar, or companion crops that deter nematodes.
- Long-term: Over time, with repeated applications/maintenance, you’ll build stronger soil biology, fewer nematode issues, healthier roots, and more resilient plants.
From Problem to Resilience
Root-knot nematodes can be a vexing underground foe, especially in warm regions like we have here in North Central Florida. You don’t have to fight them with brute chemical force. Instead, by adopting a biological approach — using Trichoderma as a major ally in your soil-health toolkit — you move toward resilience, regeneration, and greater system-health.
By reducing nematode pressure, improving root vigor, enhancing microbial diversity, and supporting your plantings early with the right fungal help, you make your food-forest ecosystem stronger rather than simply battling pests. In the long run, that’s a far more sustainable path—and it aligns with your permaculture vision.
Copyright © 2025 Fruitful Food Forestry & Lauren Lynch. No portion of the original content on this website may be reproduced, in any language, without express written consent.
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